INABIS '98 - Mitogen-Activated Protein Kinase Is Involved In The Pathogenesis Of Cerebral Vasospasm

Mitogen-Activated Protein Kinase Is Involved In The Pathogenesis Of Cerebral Vasospasm
Zubkov, A.Y. (Department of Neurosurgery, University of Mississippi Medical Center, USA)
Ogihara, K. (Department of Neurosurgery, University of Mississippi Medical Center, USA)
Tumu, P. (Department of Neurosurgery, University of Mississippi Medical Center, USA)
Lewis, A.I. (Department of Neurosurgery, University of Mississippi Medical Center, USA)
Parent, A.D. (Department of Neurosurgery, University of Mississippi Medical Center, USA)
Zhang, J. (Department of Neurosurgery, University of Mississippi Medical Center, USA)

Contact Person: Alexander Y. Zubkov, MD (azubkov@neurosurgery.umsmed.edu)

Abstract

Cerebral vasospasm is a major cause of mortality and morbidity in the patients suffering from subarachnoid hemorrhage (SAH). Pathogenetic mechanism on the cell signal transduction levels were not completely clarified. MAP kinase plays an important role in smooth muscle contraction to arterial hemolysate. Isometric tension studies and western blot were used for this purpose. MAP kinase inhibitor PD98059 was able to significantly inhibit contraction to hemolysate and relax the artery, already contracted with 10% hemolysate. Western blot studies showed that MAP kinase was activated within 1 minute after stimulation with 10% hemolysate and maintained activity for at least 120 minutes. The peak of MAP kinase activation was at 10 minutes of incubation with 10% hemolysate. Conclusion: MAP kinase appears to play an important role in cerebral smooth muscle contraction. It may be a key substrate leading to pathologic changes in the cerebral arteries affected by cerebral vasospasm.

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Presentation Number SAalexander y. zubkov0457
Keywords: mitogen, protein kinase, pathogenesis, vasospasm, smooth muscle

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Zubkov, A.Y.; Ogihara, K.; Tumu, P.; Lewis, A.I.; (1998). Mitogen-Activated Protein Kinase Is Involved In The Pathogenesis Of Cerebral Vasospasm. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Invited Symposium. Available at URL http://www.mcmaster.ca/inabis98/zhang/zubkov0457/index.html