Abstract

SERCA pumps

Characteristics of SERCA3

Molecular Characterization of SERCA3

Alternative Splicing of SERCA3 pre-mRNA

References

Discussion
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In vertebrates, the transcription of three distinct genes (SERCA1, 2, and 3) and alternative processing of the 3'-end of their pre-mRNA contribute to the isoform diversity of the SERCA family. Our recent studies concerning the expression and characterization of the SERCA3 gene provided the following insights: I) SERCA3 and SERCA2b are co-expressed both at protein and mRNA level in certain cell types and cell lines from human and rat (Wuytack et al., 1994, J. Biol. Chem., 269, 1410-1416). II) Human SERCA3 cDNA and genomic clones were isolated and the gene (ATP2A3) was mapped to human chromosomal position 17p13.3 (Dode et al., 1996, Biochem. J., 318, 689-699). III) The human SERCA3 gene (50 kb) consists of 22 exons and its transcription is driven by a GC-rich TATA-less promoter (the promoter region from -135 to -31, containing six putative Sp1 sites, is critical for the transcription of SERCA3 in Jurkat cells); SERCA3a, 3b, and 3c isoforms presenting different C-termini are generated, respectively, by exclusion, partial and total retention of exon 21 in both human and mouse genes. Mouse SERCA3 isoforms overexpressed in COS cells are able to function as active pumps, but with somewhat different affinities for Ca2+ (SERCA3a > SERCA3b and SERCA3c) (Dode et al., 1998, 273, 13982-13994).

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Presentation Number SAwuytack0580
Keywords: SERCA3, calcium, transport, ATPase