Abstract

Introduction

Materials & Methods

Results

Discussion & Conclusion

References

Discussion
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Samples from bladder dome and bladder neck of human and guinea-pig and from prostate (TUR-P) were cut into small pieces (0.5 mm diameter). Those explants were cultured on plastic dishes (35 mm), which were used pure or coated either with collagen A, laminin or poly-l-lysine in a humidified atmosphere with 5% CO2 at 37C.

To characterize the cell population we did immunocytochemical stainings for various antigens and BrdU pulse labelling to examine cell cycle. For calcium-imaging experiments subconfluent cultures were incubated in 2.5 M fura-2 (Molecular Probes) for 30 min at 37C. Following a wash in oxygened Krebs-Ringer cells were stimulated with different neurotransmitters and changes in the intracellular calcium concentration were monitored by ratio-fluorimetry (T.I.L.L. Photonics, Martinsried). Several experiments were done after pre-incubation of the cells with appropriate blocking agents and thereafter stimulation in the presence of the blocking agents to further characterize the involved transmitter receptors.

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