Abstract

Introduction

Materials & Methods

Results

Discussion & Conclusion

References

Discussion
Board

Ando, J., Tsuboi, H., Korenaga, R., Takada, Y., Toyama-Sorimachi, N., Miyasaka, M., and Kamiya, A. (1994). Shear stress inhibits adhesion of cultured mouse endothelial cells to lymphocytes by downregulating VCAM-1 expression. Am J Physiol, 267, C679-C687.

Andrews, N.C., and Faller, D.V. (1991). A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells. Nucleic Acids Res, 19, 2499

Asakura, T., and Karino, T. (1990). Flow patterns and spatial distribution of atherosclerotic lesions in human coronary arteries. Circ Res, 66, 1045-1066.

Chappell, D.C. Varner, S.E., Nerem, R.M.., Medford, R.M., Alexander, R.W. (1998) Oscillatory shear stress stimulates adhesion molecule expression in cultured human endothelium, Circ. Res. 82, 532-539.

Chien, S., Li, S., and Shyy, J.Y.J. (1998). Effects of mechanical forces on signal transduction and gene expression in endothelial cells. Hypertension 31, 62-169..

Christensen, C.W., Samet, M.M., Chick, D.M., and Lelk es, P.I. (1992). Experimental studies of pulsatile flow and endothelial-cell adaptation in ventricle-shaped cell culture chambers. ASAIO J, 38, M501-M506.

Collins, T. (1993). Biology of disease: endothelial nuclear factor-kB and the initiation of the atherosclerotic lesion. Lab Invest, 68, 499-508.

Collins, T., Palmer, H.J., Whitley, M.Z., Neish, A.S., and Williams, A.J. (1993). A common theme in endothelial activation. Insights from the structural analysis of the genes for E-selectin and VCAM-1. TCM, 3, 92-97.

Collins, T., Read, M.A., Neish, A.S., Whitley, M.Z., Thanos, D., and Maniatis, T. (1995). Transcriptional regulation of endothelial cell adhesion molecules:NF-kB and cytokine-inducible enhancers. FASEB J, 9, 899-909.

De Keulenaer, G.W., Chappell, D.C., Ishizaka, N., Nerem, R.M., Alexander, R.W., andGriendling, K.K. (1998) Oscillatory and steady laminar shear stress differentially affect human endothelial redox state: Role of a superoxide-producing NADH oxidase, Circ. Res. 82, 1094-1101.

Frang os, J.A. (1993). Physical forces and the mammalian cell. New York: Academic Press.

Gimbrone, M.A., Jr., Resnick, N., Nagel, T., Khachigian, L.M., Collins, T., and Topper, J.N. (1997). Hemodynamics, endothelial gene expression, amd atherogenesis. Ann N Y Acad Sci, 811, 1-11.

Gosh, S., May, M.J., and Kopp, E.B. (1998). NF-kappaB and rel proteins: evolutionary conserved mediators of immune responses. Annu Rev Immunol 16, 225-260.

Hanks, S.K., Calalb, M.B., Harper, M.C., and Patel, S.K. (1992). Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin. Proc Natl Acad Sci USA, 89, 8487-8491.

Ishida, T., Peterson, T.E., Kovach, N.L., and Berk, B.C. (1996). MAP kinase activation by flow in endothelial cells - Role of beta1 integrins and tyrosine kinases. Circ Res, 79, 310-316.

Kanda, K., Hayman, G.T., Silverman, M.D., and Lelkes, P.I. (1998). Comparison of ICAM-1 and VCAM-1 expression in various human endothelial cell types and smooth muscle cells. Endothelium,6,33 -44.

Kettlun C.S, Samet, M.M. and Lelkes, P.I. (1996) Pulsatile, disturbed flow enhances VCAM-1 expression in cultured human endothelial cells, J. Vasc. Res. 33, S49.

Khachigian, L.M., Resnick, N., Gimbrone, M.A., Jr., and Collins, T. (1995). Nuclear factor-kappa B interacts functionally with the platelet-derived growth factor B-chain shear-stress response element in vascular endothelial cells exposed to fluid shear stress. J Clin Invest, 96, 1169-1175.

Khachigian, L.M., Fries, J.W.U., Benz, M.W., Bonthron, D.T., and Collins, T. (1994). Novel cis-acting elements in the human platelet-derived growth factor B-chain core promoter that mediate gene expression in cultured vascular endothelial cells. J Biol Chem, 269, 22647-22656.

Khachigian, L.M., Lindner, V., Williams, A.J., and Collins, T. (1996). Egr-1-induced endothelial gene expression: a common theme in vascular injury. Science, 271, 1427-1431.

Khachigian, L.M., Williams, A.J., and Collins, T. (1995). Interplay of Sp1 and Egr-1 in the proximal plat elet-derived growth factor A-chain promoter in cultured vascular endothelial cells. J Biol Chem, 270, 27679-27686.

Korenaga, R., Ando, J., Kosaki, K., Isshiki, M., Takada, Y., and Kamiya, A. (1997). A double AP-1 consensus element is essential for the down regulation of VCAM-1 gene expression by shear stress in murine endothelial cells. Am J Phys, in press

Lan, Q., Mercurius, K.O., and Davies, P.F. (1994). Stimulation of transcription factors NFkappaB and AP1 in endothelial cells subjected to shear stress. Biochem Biophys Res Commun, 201, 950-956.

Lelkes, P.I., ed. (1999) Mechanical Forces and the Endothelium, Harwood Academic Publishers, London, U.K. in press

Lelkes, P.I., and Samet, M.M. (1991). Pulsatile flow and EC morphology in a VAD-like chamber. ASAIO J, 37, M315-M316.

Li, H., Cybulsky, M.I., Gimbrone, M.A., Jr., and Libby, P. (1993). An atherogenic diet rapidly induces VCAM-1, a cytokine-regulatable mononuclear leukocyte adhesion molecule, in rabbit aortic endothelium. Arterioscler Thromb, 13, 197-204.

Lin, M.-C., Almus-Jacobs, F., Chen, H.-H., Parry, G.C.N., Mackman, N., and Shyy, J.Y. (1997). Shear Stress induction of the tissue factor gene. J Clin Invest, 99, 737-744.

Malek, A., and Izumo, S. (1992). Physiological fluid shear stress causes downregulation of endothelin-1 mRNA in bovine aortic endothelium. Am J Physiol, 263, C389-C396.

Malek, A.M., and Izumo, S. (1996). Control of endothelial cell gene expression by flow. J Biomechanics, 28, 1515-1528.

Marui, N., Offermann, M.K., Swerlick, R., Kunsch, C., Rosen, C.A., Ahmad, M., Alexander, R.W., and Medford, R.M. (1993). Vascular cell adhesion molecule-1 (VCAM-1) gene transcription and expression are regulated through an antioxidant-sensitive mechanism in human vascular endothelial cells. J Clin Invest, 92, 1866-1874.

Nikolaychik, V.V., Samet, M.M., and Lelkes, P.I. (1994). A new, cryoprecipitate-based coating for improved endothelial cell attachment and growth on medical grade artificial surfaces. ASAIO J, 40, M846-M852.

Papadimit riou, E., and Lelkes, P.I. (1993). Measurement of cell numbers in microtiter culture plates using the fluorescent dye Hoechst 33258. J Immunol Methods, 162, 41-45.

Rasouly, D., Shavit, D., Zuniga, R., Elejalde, R.B., Unsworth, B.R., Yayon, A., Lazarovici, P., and Lelkes, P.I. (1996). Staurosporine induces neurite outgrowth in neuronal hybrids (PC12EN) lacking NGF receptors. J Cell Biochemistry, 62, 356-371.

Read, M.A., Neish, A.S., Gerritsen, M.E., and Collins, T. (1996). Postinduction transcriptional repression of E-selectin and vascular cell adhesion molecule-1. J Immun, 157, 3472-3479.

Read, M.A., Whitley, M.Z., Williams, A.J., and Collins, T. (1994). NF-kappaB and IkappaBalpha: an inducible regulatory system in endothelial activation. J Exp Med, 179, 503-512.

Resnick, N., Collins, T., Atkinson, W., Bonthron, B.T., Dewey, C.F., and Gimbrone, M.A., Jr. (1993). Platelet-derived growth factor B chain promoter contains a cis-acting fluid shear-stress-responsive element. Proc Natl Acad Sci USA, 90, 4591 -4595.

R. Ross, The pathogenesis of atherosclerosis: a perspective for the 1990s. (1993). Nature 362:801-809.

Rubanyi, G.M., Freay, A.D., Kauser, K., Johns, A., and Harder, D.R. (1990). Mechanoreception by the endothelium: mediators and mechanisms of pressure- and flow-induced vascular responses. Blood Vessels, 27, 246-257.

Samet, M.M., and Lelkes, P.I. (1993). Flow patterns and endothelial cell morphology in a simplified model of an artificial ventricle. Cell Biophys, 23, 139-163.

Samet, M.M., and Lelkes, P.I. (1994). Pulsatile flow and endothelial cell morphology in a cell culture chamber model of an artificial cardiac ventricle. J Biomech Eng, 116, 369-371.

Samet, M.M., and Lelkes, P.I. (1999) The hemodynamic environment of the endothelium in vivo and it simulation in vitro IN: Mechanical forces and the endothelium (Lelkes, P.I., ed.), Harwood Academic Press, London, U.K., in press.

Schwartz, M.A., and Ingber, D.E. (1994). Integrating with integrins. Mol Biol Cell, 5, 389-393.

Sumpio, B.E., Du, W., Galagher, G., Wang, X.-J., Khachigian, L.M., Collins, T., and Gimbrone, M.A.,Jr. (1998) Regulation of PDGF-B in endothelial cells exposed to cyclic strain. Arterioscler.Thromb.Vasc.Biol. 18:349-355,

Shyy, J.Y.-J., Li, Y.-S., Lin, M.-C., Chen, W., Yuan, S., Usami, S., and Chien, S. (1995). Multiple cis-elements mediate shear stress-induced gene expression. J Biomechanics, 28, 1451-1457.

Shyy, J.Y.-J. and Chien, S. Role of integrins in cellular responses to mechanical stress and adhesion. (1997) Curr.Opin.Cell Biol. 9,707-713.

Silverman, M.D., Manolopoulos, V.G., Unsworth, B.R., and Lelkes, P.I. (1996). Tissue factor expression is differentially modulated by cyclic mechanical strain in various human endothelial cells. Blood Coagul Fibrinolysis, 7, 281-288.

Sumpio, B.E.(ed.). (1993). Hemodynamic forces and vascular cell biology. Austin: R. G. Landes Company.

Sumpio, B.E., Du, W., Galagher, G., Wang, X.-J., Khachigian, L.M., Collins, T., and Gimbrone, M.A.,Jr. (1998) Regulation of PDGF-B in endothelial cells exposed to cyclic strain. Arterioscler. Thromb. Vasc. Biol. 18, 349-355.

Takahashi, M., and Berk, B.C. (1996). Mitogen-activated protein kinase (ERK1/2) activation by shear stress and adhesio n in endothelial cells - Essential role for a herbimycin-sensitive kinase. J Clin Invest, 98, 2623-2631.

Wung, B.S., Cheng, J.J., Hsieh, H.J., Shyy, Y.J., and Wang, D.L. (1997). Cyclic strain-induced monocyte chemotactic protein-1 gene expression in endothelial cells involves reactive oxygen species activation of activator protein 1. Circ Res, 81, 1-7.

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