Abstract

Introduction

Materials & Methods

Results

Discussion & Conclusion

References

Discussion
Board

Sensor devices are rapidly replacing conventional analytical techniques, primarily due to the characteristics of small size, rapid response, low cost and disposability. We are developing an immunosensor device with the potential to detect and measure many analytes. Initial studies are being based on the detection of biotin. The sensor is based on the electropolymerisation of aniline on the surface of a miniature screen-printed carbon electrode. Antibody to biotin has been electrostatically attached to the resulting polyaniline surface by reduction and re-oxidation. A competitive immunoassay format was developed using free biotin and a biotin-horseradish peroxidase conjugate. Introduction of the substrate, hydrogen peroxide then results in an increase in the steady state current due to the catalysis of hydrogen peroxide by horseradish-peroxidase. We hope to extend this system to environmental and biomedical applications.

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Poster Number PAkillard0115
Keywords: immunosensor, electrode, polyaniline, screen-printed, biotin