Abstract

Introduction

Materials & Methods

Results

Discussion & Conclusion

References

Discussion
Board

The main immunoglobulin fraction of fowls is called IgY, in order to distinguish it from the mammalian IgG. There are some crucial points of difference between IgG and IgY. Avian IgY is more resistant to temperature, pH and ion strength of environment than IgG. IgY has no reactivity to mammalian auto-antibodies, Fc-receptors or RBCs. In mammalian systems, these cross-reactions can be eliminated using avian-originated antibodies (ABs). Serum IgY is identical to yolk AB, as has been proven in comparative studies. The isolation and purification for further different uses of AB has great potential. The extraction and purification of serum-derived immunoglobulin fraction (IgY) in the egg yolks of domestic hens and Japanese quail hens is described herein. The method of extraction consists of four steps:1. Water dilution of egg yolks; 2. Precipitation of lipids with dextran sulphate (DS) containing CaCl₂, then centrifugation; 3. Precipitation of proteins by sodium sulphate; and 4. Dialysis of precipitate against PBS. The purification of dialyzed samples was taken by chromatography using anion-exchange (DEAE-Seplacel) and gel filtration (Ultrogel AcA 22) techniques. 35% and 27% of the yield of IgY were given in the case of chicken and quail eggs. These relatively poor yields are not judged as serious because of the high quantity of AB source (i.e. egg yolk). With regard to animal welfare (i.e. bleeding), ABs purified from the egg yolks of immunized chickens are an attractive alternative to AB raised from the serum of mammals. Avian egg yolk ABs can play an increasing role as an alternative to classic (mammalian) ABs. They can be used in immunodiagnostics and therapy as well.

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Poster Number PAszabo0509
Keywords: antibodies (IgY), chicken, quail, egg yolk, chromatography