Abstract

Introduction

Materials & Methods

Results

Discussion & Conclusion

References

Discussion
Board

HLA-A2402 restricted and carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes (CTLs) were induced by culturing human peripheral blood mononuclear cells (PBMC) on formalin-fixed autologous adhesive PBMC that have been loaded with CEA-bound latex-beads. The CTLs killed the CEA-producing HLA-type matched cancer cells, but not the non producers of CEA, at an effector/target ratio of 10 within 24 hours. On the basis of available HLA-A24 binding peptides, we have also attempted to identify the epitope peptide recognized by the CTLs. The peptide CEA652(9), TYACFVSNL, stimulated the most strongly the CTLs when pulsed on HLA-A2402 expressing target cells. Other 9 peptides so far tested were also active, but less efficiently, to the CTLs. The CTLs failed to kill target cells pulsed with HLA-A2 binding CEA peptide, CAP-1. The CTLs were also generated on the fixed adherent cells previously pulsed with the peptide CEA652(9). Cytotoxic activity of the CTLs was inhibited by monoclonal antibodies against CD3, CD8, and MHC-class I molecules. These results suggest that human autologous CTLs will be inducible on the autologous fixed PBMC without use of the cultured target cancer cells if tumor antigenic protein is available.

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Poster Number PAkim0330
Keywords: CEA, CTL