Immunology & Immunological Disorders Poster Session

Re: Poster 327

M.Kohyama
kohyama@rtc.riken.go.jp (kohyama masako)

On Sat Dec 5, Grover wrote
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>Dr. Kohiyama:Hope you are enjoying the meeting. Great poster. I am bit confused. In your methods you indicated "@PBMC from patient A were stimulated with autologous tumor cells (TKB9) in medium containing IL-2, or an IL-cocktail consisting of IL-1, -2, -4, and -6, with or without IL-18, for 28 days@PBMC from patient A were stimulated with autologous tumor cells (TKB9) in medium containing IL-2, or an IL-cocktail consisting of IL-1, -2, -4, and -6, with or without IL-18, for 28 days." With this experiment, how does one conclude that IL-18 effects were direct on the cells rather than on the cytokine molecules themselves or on some signal produced by the cocktail cytokines? This brings to my second question, how do you think IL-18 exerts its effect?

Dear Dr.Grover
I can not answer your first question. Because, I don't know the example that cytokines interact directly to each other. Please teach me some example about that.
IL-18 formaly designated IFN-g inducing factor. So the direct activation of CD8+ CTL by IL-18 may be dependent on an increase in levels of IFN-g.

Thu Dec 10