Immunology & Immunological Disorders Poster Session

Poster 327

Dr. Kohiyama:Hope you are enjoying the meeting. Great poster. I am bit confused. In your methods you indicated "@PBMC from patient A were stimulated with autologous tumor cells (TKB9) in medium containing IL-2, or an IL-cocktail consisting of IL-1, -2, -4, and -6, with or without IL-18, for 28 days@PBMC from patient A were stimulated with autologous tumor cells (TKB9) in medium containing IL-2, or an IL-cocktail consisting of IL-1, -2, -4, and -6, with or without IL-18, for 28 days." With this experiment, how does one conclude that IL-18 effects were direct on the cells rather than on the cytokine molecules themselves or on some signal produced by the cocktail cytokines? This brings to my second question, how do you think IL-18 exerts its effect?

Sat Dec 5