Cell Biology Poster Session
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Hattori, K. (Dept. of Pathol., Northwestern Univ. Med. Sch, Chicago)
Uchida, D. (Second Dept of Oral and Maxillofacial Surg., Tokushima Univ. Sch. of Dent., Japan)
Hino, S. (Second Dept of Oral and Maxillofacial Surg., Tokushima Univ. Sch. of Dent., Japan)
Omotehara, F. (Second Dept of Oral and Maxillofacial Surg., Tokushima Univ. Sch. of Dent., Japan)
Sato, M. (Second Dept of Oral and Maxillofacial Surg., Tokushima Univ. Sch. of Dent., Japan)
Oyasu, R. (Dept. of Pathol., Northwestern Univ. Med. Sch, USA)
Abstract
EGF is a potent mitogen, and its action is mediated by MAPK in several cell types. Recently, it is reported that EGF activates STATs, induces the expression of p21waf1, and inhibits the growth of several cancer cells. In this study, we examined the growth of bladder (T24 and RT4) and epidermal (A431) cancer cells, and immortalized human urothelial cells (1T-1, 1T-2, and 1T-3) after EGF treatment, and compared their growth with the activities of MAPK and STAT, and the induction of p21waf1. EGF inhibited the growth of A431 and T24, but not of RT4. EGF stimulated the growth of 1T-1 strongly, and 1T-3 slightly, but not all of 1T-2. EGF activated the MAPK strongly in 1T-1 and A431, and slightly in RT4, but marginally in 1T-2, 1T-3 and T24. Futhermore, we detected the activation of STAT1 in A431 and 1T-3, and STAT1 and STAT3 in T24 after EGF treatment. EGF also enhanced the expression of p21waf1 mRNA in A431, T24, 1T-2, and 1T-3. These results suggest that balance between the STAT-induced p21waf1 and MAPK activities regulates the growth of human bladder cell lines after EGF treatment.
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Poster Number PAkawamata0333
Keywords: stat, mapk, bladder cancer, egf
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