Abstract

Introduction

Materials & Methods

Results

Discussion & Conclusion

References

Discussion
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Novel type of antigen preparation - neuraminidase-treated desialylated normal membranes - was applied with known test for lymphocyte sensitization in cancer patients - electrophoretic mobility of the indicator cells (EM-test). Previously in the rat and mouse experimental models it was shown that the existance of the desialilated antigens in the organism may serve as a marker of the malignancy and the lowest level of the positive reaction was approximately 10 000 transformed cells. The determination of normal (obtained from non-malignant breast tissue) desialylated antigens in primary breast cancer patients has the sensitivity of 83% and specificity of 90%. While determining the prognostic ability of the EM-test with breast cancer patients after surgery and radio- chemotherapy the correlation between antigen's sensitization and recurrence was revealed in 81% patients, sometimes in terms 1-3 years before metastatic spread. Though the test is rather time- and labour- consuming, it is perspective and may be applied to use with ELISA reader.

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Poster Number PAnovichenko0442
Keywords: breast cancer, immunodetection, EM-test, neuraminidase, desialylation