Pharmacology & Toxicology Poster Session

Grover
groverak@fhs.mcmaster.ca

On Fri Dec 11, Albert Woelfler wrote
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>On Sun Dec 6, Grover wrote
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>>Dr. Woelfler: Great presentation.  Hope you have fun at the meeting.  I had never thought of melatonin as a calmodulin antagonist and your results show that it is not.  I am not sure what concentrations of TFP you used as TFP itself can have membrane effets unrelated to calmodulin.
>>

>Dear Dr Grover,

>Melatonin has been reported to bind to the calcium/calmodulin complex with high affinity (Life Sci (1993)53: 201-207; FASEB (1998) 13: 1401-1408) and it has been shown to inhibit Ca/calmodulin -dependent enzymes in presence of Ca/calmodulin in biochemical assays (Brain Res (1991) 557: 289-292; Biochim Biophys Acta (1996) 1290: 191-196). However, whether melatonin can bind to calmodulin and inhibit the activation of calmodulin-dependent enzymes within cells is  not known.
>We used 1 to 10µM TFP. At this concentrations TFP has been shown to be a potent calmodulin inhibitor.

>Regards,
>Albert Woelfler