Immunology & Immunological Disorders Poster Session

Poster 456

Dr. Fagan: Hope you are enjoying the meeting. Great poster.Hope you are enjoying the meeting. Great poster. I am intrigued by the composition of your PBS-azide. followed by a wash with PBS-azide: 0.01 M sodium phosphate and 0.015 M sodium chloride, containing 0.01g/ml of sodium azide, pH 7.4, at 4o C). First this is hypotonic and would it not swell the cells. Second, I understood that azide would inhibit any mitochondrial activity of the cell. Anyways, I was intrigued by the effect of K-oxalate. What sort of concentration of K-oxalate did you use? I would expect high concentrations of this agent to produce cell depolarization and hence cell activation.

Sat Dec 5