Novel Cellular and Molecular Mechanisms in Allergic Inflammation

Re: Start-up comment on Grayson et al presentation, ,

On Sat Dec 5, Judah Denburg wrote
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>To Bruce Bochner and colleagues:

>Is alpha d found on immature eosinophils or on basophils?
>What interaction is there between IL-5R and alpha d expression? Presumably IL-5 would induce alpha d by IL-5R-mediated events.
>Judah Denburg

We did not study the expression of alpha-d on immature cell types; however, it seems reasonable to assume that alpha-d expression is present as these cell types mature, much like CD11b/CD18. Indeed, Van der Vieren, et. al. have shown that the promyelomonocytic cell line HL60 expresses alpha-d and this expression is not affected by DMSO treatment (Immunity. 1995 Dec;3(6):683-90.).

In our initial studies we did not characterize further the induction of alpha-d expression by IL-5. Most likely this effect is mediated through the IL-5R; however, we cannot rule out a more indirect pathway.

Mitch Grayson

Mon Dec 7